ABSTRACT
The methanolic extract of dried fruit pulp of Citrullus colocynthis [Cucurbitaceae] has been studied with respect to antimicrobial and toxicological properties. The antimicrobial profile was investigated against thirty bacterial isolates [10 Gram +ve and 20Gram-ve] and five fungal species. None of the bacterial or fungal culture used in the study showed sensitivity against the extract. Acute toxicity studies carried out in Albino mice NMRI indicated the highly toxic nature of the colocynth. A very significant decrease in body weight of test animals was noted at P<0.05. The LD[50] was calculated as 1000mg/kg body weight. Within four days of experimentation mortality was 100%. Histopathological studies confirmed the toxic nature of extract. Gross changes in histology of Heart, Liver and Kidneys were noted. Section of spleen did not exhibit any abnormality
ABSTRACT
Neurotoxicants and environmental pollutants cause neuronal damage and may damage myelin sheath. Since Methyl cobalamine [B12] has effect on myelin sheath formation it was hypothesized that B12 administration in case of nerve damage by some neurotoxicants may be protected or corrected by B12 administration. [Braun, 1977 and Braun, 1980]. For generations physicians have recommended B-vitamins especially B12 for neuropsychiatric symptoms. In present study neuroprotective role of B12 is assessed. Present work was planned to evaluate the therapeutic or prophylactic role of B12 in nerve damage by neurotoxicant i.e. glutamic acid. Present study revealed no significant neuroprotection or defensive regimen offered by B12 for neurotoxic damage. Glutamic acid is probably affecting neurons or dentritis sparing myelin sheath. Neuroprotection expected by B12 may be applied only to myelin sheath